Zdenek Ondrasek produced a late debut winner as Czech Republic came from behind to take a shock 2-1 home win over England.
Harry Kane gave England an early lead from the penalty spot before Jakub Brabec pulled the hosts level just minutes later from a tap-in at the back post from a corner kick.
A tight tussle then endured with both teams wasting chances until Ondrasek rifled in a low pass from Lukas Masopust to send the Czechs level with England in Euro 2020 Qualifying Group A on 12 points apiece.
This result also marks England’s first qualifier defeat in ten years of which they lost 1-0 in Ukraine back in October 2009.
Czech Republic made the better start from kick-off as a cross from Jan Boril found Patrik Schick, who could only flick it wide to spare England’s blushes.
Raheem Sterling soon played a wonderful overlap up the left before cutting into the box, only for Masopust to bring him down and referee – Damir Skomina paused before pointing to the penalty spot.
Kane stepped up and calmly dispatched the fifth minute penalty down the middle as Tomas Vaclik dived to his right, sending England on their way to what would of appeared to be a routine victory after thrashing their hosts 4-0 at Wembley in March.
The Locomotives however came back and earned a corner three minutes later when Vladmir Coufal’s shot was deflected over for a corner. The resulting delivery found Ondrej Celustka who teed up Barbec to poke in their equaliser at the back post.
Both teams then had chances to find their second goal before half-time with the hosts coming closest when Schick headed a 32nd minute free-kick on goal, only for his effort to be straight at Jordan Pickford who made a comfortable save.
It proved to be more of the same after the break as Czech Republic pressed England more, although Sterling did have the best early chance from another overlap after 57 minutes but Vaclik timed a brilliant block to deny the Manchester City winger.
Ondrasek then grabbed his debut goal five minutes from time as an attack was triggered up their right, with Masopust teeing up the unmarked FC Dallas forward to rifle low into the bottom right corner to complete the unlikiest of comeback wins.
England however remain top of the group on goal difference and visit Bulgaria on Monday night, where they could secure their spot at the main tournament next summer if they win and Kosovo doesn’t beat Montenegro.